Figure 1

Cortical and hippocampal number of neuronal and non-neuronal cells from the exercise (EX) and control (CTL) groups evaluated using the isotropic fractionator method at 0 (P60), 30 (P90) and 60 (P120) days after the last exercise session (n = 5–6 in each age and group). Representative fluorescence images of neuronal and non-neuronal nuclei stained with 4′-6-diamidino-2-phenylindole dihydrochloride (DAPI) (blue), neuron-specific nuclear protein (NeuN) (red) and DAPI + NeuN (pink in merged image) can be seen on the left. Scale bar = 25 μm. Significant difference compared with P60a, P90b, P120c, or CTL group* (p < 0.05 by two-way ANOVA and Tukey post-test).