Figure 3

Increased BrdU-incorporation 36 h post-PH in Epac1^−/−, Epac2^−/− and Epac1/2^−/− mice. (a) BrdU incorporation was analyzed in wt, Epac1^−/−, Epac2^−/− and Epac1/2^−/−  mice post-PH for the indicated time points. Data is shown as box and whiskers with median and min to max whiskers, n = 2–13 mice/group (6–13 mice/group at 26 h and 36 h). (b) Quantification of DAPI staining pre-PH, 26 h and 36 h post-PH. Data is shown as mean ± SD, n = 3–9 mice/group. Two-way ANOVA with Bonferroni’s adjustment for multiple comparisons was used to determine statistical differences between genotypes within each time point. *p < 0.05, ***p < 0.001 and ****p < 0.0001. F-statistics; (a) F(9,104) = 5.331, p < 0.0001 and (b) F(6,55) = 1.436, p = 0.2176. (a) 36 h post-PH (mean ± SD): wt: 15.04 ± 6.61; Epac1^−/−: 27.97 ± 10.22; Epac2^−/−: 36.57± 15.32; Epac1/2^−/−: 34.86 ± 9.37. (b): 36 h post-PH (mean ± SD): wt: 49.6 ± 4.1; Epac1^−/−: 57.8 ± 1.9; Epac2^−/−: 58.4 ± 2.4; Epac1/2^−/−: 58.9 ± 3.2.