Figure 11
From: Cold Atmospheric Plasma and Plasma-Activated Medium Trigger RONS-Based Tumor Cell Apoptosis
Elucidation of the action of primary singlet oxygen (1O2) directed towards membrane-associated catalase. (A) The NOX1 inhibitor AEBSF prevents the generation of secondary singlet oxygen (1O2). The singlet oxygen scavenger histidine (HIS) completely prevents inactivation of catalase by primary singlet oxygen (1O2) generated from long-lived species in PAM and thus ensures that singlet oxygen is the cause of inactivation in this scenario. (B) AEBSF prevents the generation of secondary singlet oxygen (1O2). The addition of either the catalase mimetic EUK-134, or the hydroxyl radical scavenger mannitol (MANN) or the ONOO− decomposition catalyst FeTPPS prevent the generation of primary singlet oxygen (1O2) derived from long-lived species in CAP-treated medium (#11-#19). Inhibition by these compounds discriminates between primary singlet oxygen generated through interaction between long-lived species in PAM and primary singlet oxygen that is directly derived from the gaseous phase of CAP. Supplementary Figs 11–13 show details of the detection of singlet oxygen directly derived from CAP.
