Table 1 Summary of enzyme inhibitors, reactive species scavengers, reactive species donors, mimetics, and antibodies used in the present study to elucidate apoptotic and protective mechanisms.
From: Cold Atmospheric Plasma and Plasma-Activated Medium Trigger RONS-Based Tumor Cell Apoptosis
Purpose | Compound name | Compound abbreviation and standard working concentration |
|---|---|---|
Singlet oxygen scavenger | Histidine | HIS 2 mM |
Peroxynitrite decomposition catalyst | 5-, 10-, 15-, 20-Tetrakis(4-sulfonatophenyl)porphyrinato iron(III) chloride | FeTPPS 25 µM |
NOX1 inhibitor | 4-(2-Aminoethyl) benzenesulfonyl fluoride | AEBSF 100 µM |
HOCl scavenger | Taurine | TAU 50 mM |
Aquaporin inhibitor | AgNO3 | Ag+ 5 µM |
Catalase inhibitor | 3-aminotriazole | 3-AT 25 mM |
Catalase donation (bovine liver catalase) | Catalase | CAT 10 - 1000 U/ml |
glutathione synthesis inhibitor | Buthionine sulfoximine | BSO 10 - 50 µM |
⋅OH scavenger | Manitol | MANN 20 mM |
⋅OH scavenger | Dimethylthiourea | DMTU 20 mM |
NO donor | Dieethylamine NONOate | DEA NONOate 0.5 mM |
HOCl donor | Sodium oxychloride | NaOCl as indicated |
Generation of H2O2 | Glucose oxidase | GOX as indicated |
Nitric Oxide Synthase inhibitor | N-omega-nitro-L-arginine methylester hydrochloride | L-NAME 2.4 mM |
Proton pump inhibitor | Omeprazole | |
Peroxidase inhibitor | 4-Aminobenzoyl hydrazide | ABH 150 µM |
Caspase-3 inhibitor | Z-DEVD-FMK 50 µM | |
Caspase-8 inhibitor | Z-IETD-FMK 25 µM | |
Caspase-9 inhibitor | Z-LEHD-FMK 25 µM | |
SOD mimetics | Mn(III) 5,10,15,20-tetrakis(N-methylpyridinium-2-yl)porphyrin and Mn (III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin | MnTM-2PyP and MnTE-2-PyP 20 µM |
Mn-SOD donation (E. coli) | Manganese superoxide dismutase | Mn-SOD 100 U/ml |
ONOO− decomposition catalyst and O2− scavenger | Fe(III)tetrakis(1-methyl-4-pyridyl)porphyrin pentachlorideporphyrin pentachloride | FeTMPyP 25 µM |
Catalase mimetic | chloro([2,2′-[1,2-ethanediylbis[(nitrilo-κN)methylidyne]]bis[6-methoxyphenolato-κO]]]-manganese | EUK-134 20 µM |
Antibody for human superoxide dismutase (SOD) | cb 0989 (binding and neutralizing) cb 0987 (binding without neutralization) |
