Figure 4

IL-10 differentially modulates the cytokine production of activated ILC2s despite expression of IL-10R by all ILC subsets. (A) Sorted ILC subsets (n = 9) and PBMCs (n = 7) were isolated from healthy volunteers and assessed for expression of IL-10R mRNA by qPCR. Data are shown as a combination of three independent experiments. (B,C) IL-10R expression on circulating monocytes and ILC subsets was assessed in the whole blood of healthy volunteers (n = 10) using by flow cytometry for (B) surface expression and (C) per cell intensity assessed by geometric mean fluorescence intensity (geometric MFI). (D,E) ILC1 (n = 13) and ILC2 (n = 10–11) subsets were sorted from healthy donors and stimulated with activating cytokines (IL-12/IL-15 for ILC1s and IL/25/IL-33 for ILC2s) in the presence or absence of 50 ng/mL of IL-10 for 5 days at 2,000 cells/well. The culture supernatants were assessed for production of (D) ILC1 and (E) ILC2 signature cytokines. Paired comparison of stimulated and stimulated + IL-10 conditions was accomplished with the Wilcoxon test and levels of significance were indicated by: ***p < 0.0001, *p < 0.05 and ns, not significant.