Figure 18

Dependency of the induction of bystander effect inducing potential on the generation of secondary singlet oxygen (¹O₂). The experiment described in Fig. 12 is presented here, with the addition that 100 µM AEBSF had been present during the time of contact between PAM and cells. In this way the generation of secondary ¹O₂ was prevented specifically during the time of contact, but not after the washing step and during final incubation. The separation of the data into two figures has been chosen to allow an easier evaluation by the reader. The results show the dominating effect of secondary ¹O₂ generation for the induction of bystander effect inducing potential, on top of the initial effect of primary ¹O₂ from PAM.