Figure 22 | Scientific Reports

Figure 22

From: Dynamics of Singlet Oxygen-Triggered, RONS-Based Apoptosis Induction after Treatment of Tumor Cells with Cold Atmospheric Plasma or Plasma-Activated Medium

Figure 22

Bystander signaling is hampered through inhibition of NOX1. The presence of the NOX1 inhibitor AEBSF during CAP or PAM treatment prevents the generation of secondary 1O2. Therefore, only few cells will carry the “imprinted signature” of primary 1O2 action, i. e. a partially inactivated catalase population on their surface. To transmit bystander effect induction from this cell population to untreated cells requires to apply a much higher percentage of pretreated cells as under conditions without AEBSF. For this reason, the curves in the bystander experiments in this study show a strong right-ward shift in the presence of AEBSF during pretreatment and the kinetic analysis is characterized by a delay in apoptosis induction.

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