Figure 3
In vivo effects of anti-parkinsonian drugs on bone metabolism. (A) Representative μ-computed tomography (μCT) images of distal femurs of mice injected daily with saline (control, n = 8), levodopa (n = 6), pramipexole (n = 8), ropinirole (n = 9) or bromocriptine (n = 8) (upper, axial view of metaphyseal region; lower, longitudinal view). (B) Bone volume, trabecular number, trabecular thickness and degree of trabecular separation were determined by μCT analysis. (C) Bone histomorphometric analyses of tibiae obtained from control and anti-parkinsonian drug-injected mice. Representative images are shown. (D) Parameters for osteoclastic bone resorption and osteoblastic bone formation, as determined by bone morphometric analyses. (E) Serum level of homocysteine in control and anti-parkinsonian drug-injected mice determined by ELISA (n = 6). Statistical analyses were performed using ANOVA with Dunnett’s multiple-comparison test. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant. Error bars represent ± s.e.m.
