Figure 2
From: NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
Ciliogenesis is reduced in NRF2-null cells. (a) Representative immunofluorescence images for ARL13B, acetylated α-tubulin (AcTub) and γ-tubulin (γTub) in Nfe2l2+/+ and Nfe2l2−/− littermate MEFs starved 24 h to induce ciliogenesis. Nuclei were counterstained with DAPI. Scale bar, 10 μm. (b) Quantitation of percentage of ciliated cells from (a) (mean ± SEM of n = 3 independent experiments). (c) Quantitation of cilia length from (a) (mean ± SEM of n = 81–85 cilia measured per condition). (d) Representative immunofluorescence images for ARL13B, γ-tubulin and astrocyte marker GFAP in hippocampus of 3-month old Nfe2l2+/+ and Nfe2l2−/− mice. Nuclei were counterstained with DAPI. Boxed region in left is magnified on the right. Arrowheads point to cilia. Scale bars, 25 μm (left) and 10 μm (right). (e) Quantitation of percentage of ciliated cells from (d) (mean ± SEM, n = 3 mice). Asterisks in (b), (c) and (e): statistical significance in unpaired two-tailed t-tests with **p < 0.01, ***p < 0.001 or ****p < 0.0001.
