Figure 3
From: NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
Hh signaling is reduced in NRF2-null cells. (a) Nfe2l2+/+ and Nfe2l2−/− MEFs were starved 24 h in presence of DMSO or SAG (200 nM) and expression of Hh target genes Gli1 (top) and Ptch1 (bottom) was analyzed by RT-qPCR. Data shown as β-actin (Actb)-normalized relative mRNA levels (mean ± SEM, n = 8–9 independent experiments). Statistical analysis: two-way ANOVA followed by Tukey’s multiple comparisons tests. Asterisks: *p < 0.05 or ****p < 0.0001. (b) Nfe2l2 MEFs treated as in (a) were analyzed by Western blot with antibodies for GLI1, NRF2 and Lamin-B as loading control. Molecular weight markers in kilodaltons are shown on the right. Asterisk denotes non-specific band (see methods on NRF2 antibody). Uncropped blots are shown in Supplementary Fig. S12.
