Figure 4
From: NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
Hh signaling defects in NRF2-null cells are not rescued by HDAC6, PKA, mTOR or ROS inhibitors. Nfe2l2+/+ and Nfe2l2−/− MEFs were starved 24 h in presence of DMSO (vehicle), SAG (200 nM), Tubastatin A (10 μM), H89 (10 μM), Torin-1 (250 nM) or NAC (3 mM), as indicated, and expression of Hh target genes Gli1 and Ptch1 was analyzed by RT-qPCR. Data displayed as β-actin-normalized relative mRNA levels (mean ± SEM of n = 5 independent experiments). Statistical analysis: one-way ANOVA followed by Tukey’s multiple comparisons tests. Significance: *p < 0.05, ****p < 0.0001 or not significant (n.s.).
