Figure 5
From: NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
Ciliogenic and Hh pathway gene expression is reduced in NRF2-null cells. (a) Nfe2l2+/+ and Nfe2l2–/– MEFs were starved 24 h in presence of DMSO or SAG (200 nM) and expression of the indicated ciliogenic genes was analyzed by RT-qPCR. (b) Expression of the indicated Hh pathway genes was analyzed as in (a). All data in (a-b) shown as β-actin-normalized relative mRNA levels (mean ± SEM of n = 3–5 independent experiments). Statistical analysis: two-way ANOVA. Significance between genotypes: *p < 0.05, **p < 0.01, ***p < 0.001 or ****p < 0.0001. SAG treatment did not cause significant differences. (c) Putative ARE sequences were identified for the human ciliogenic and Hh pathway genes indicated on the left. Table shows genomic location and sequences of these putative AREs, as well as maximum and relative scores. Only sequences with relative score above 0.8 were considered as hits. No such hits were found for DYNC2H1, SMO, SUFU or GLI1. Optimal consensus ARE sequence is (A/G)TGACTCAGCA, according to JASPAR database (see methods for details).
