Figure 1
ELTD1 is often overexpressed and correlates with poor prognosis in glioma. (A) The expression of ELTD1 in normal brain tissues (NB) and glioma tissues (LGG: Low-grade glioma, HGG: High-grade tissue) obtained from the TCGA database. The nonparametric Mann–Whitney U-test was used. (B) The expression of ELTD1 in normal brain tissues (NB) and glioma tissues obtained from the GEO database (GSE16011, GPL8542, Affymetrix GeneChip Human Genome U133 Plus 2.0 Array, Hs133P_Hs_ENTREZG.cdf). The nonparametric Mann–Whitney U-test was used. (C) The expression of ELTD1 in normal brain tissues (NB) and glioma tissues obtained from the TCGA and GEO database. The nonparametric Mann–Whitney U-test was used. (D) The expression of ELTD1 in LGG and HGG obtained from the TCGA and GEO database. The nonparametric Mann–Whitney U-test was used. (E) Relative ELTD1 expression levels measured by RT-PCR in 26 LGG and 35 HGG. (F) Sixteen glioma tissues were assessed for ELTD1 expression by Western blotting, including four grade I, four grade II, four grade III and four grade IV. (G,H) Representative images (G) and scores (H) of the IHC of ELTD1 expression (blue) in the paraffin-embedded different grade glioma. (I–K) Kaplan–Meier curves for OS (I,K) and DFS (J) of glioma patients with low vs. high expression of ELTD1. The median ELTD1 expression was used as the cutoff value. Statistical significance was assessed using two-tailed Student’s t test (C–E, H–K) and one-way ANOVA followed by Dunnett’s tests for multiple comparisons (A,B). Scale bars: 50 μm. **p < 0.01 and ***p < 0.001.
