Figure 3
ELTD1 knockdown suppresses glioma cell proliferation, migration and invasion. (A,B) The knockdown efficiency against ELTD1 was verified by RT-PCR and Western blotting in U-87MG and U-138MG cells. (C) Growth curves between sh-NC, sh-ELTD#1 and sh-ELTD#2 by CCK-8 assay. The results are shown as the Mean ± Standard Deviation (SD) of five independent experiments. (D,E) Representative images (left panels) and histogram quantification (right panels) of the EdU (D) and colony formation assay (E) with U-87MG and U-138MG cells. (F,G) Representative images (left panels) and histogram quantification (right panels) of the Transwell migration (F) and invasion assays (G) with U-87MG and U-138MG cells. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s tests for multiple comparisons. Scale bars: 50 μm. **p < 0.01.
