Figure 5
HIF-1α is involved in ELTD1-regulated glioma cell proliferation, migration and invasion. (A) Correlation analysis of ELTD1 and HIF-1α mRNA expression by RT-PCR. (B,C) The overexpression efficiency against HIF-1α was verified by RT-PCR and Western blotting in U-87MG and U-138MG cells. (D) Growth curves between None, Vector, ELTD1 (KD), HIF-1α (OE) and ELTD1 (KD) + HIF-1α (OE) by CCK-8 assay. The results are shown as the Mean ± Standard Deviation (SD) of five independent experiments. (E,F) Representative images (left panels) and histogram quantification (right panels) of the EdU (E) and colony formation assay (F) with U-87MG and U-138MG cells. (G,H) Representative images (left panels) and histogram quantification (right panels) of the Transwell migration (G) and invasion assays (H) with U-87MG and U-138MG cells. All the results indicated that HIF-1α overexpression abrogated the effects of ELTD1 knockdown on inhibiting U-87MG and U-138MG cell proliferation (D,E), colony formation (F), migration (G) and invasion (H). Statistical significance was assessed using two-tailed Student’s t test (A,B) and one-way ANOVA followed by Dunnett’s tests for multiple comparisons (D–H). Scale bars: 50 μm. *p < 0.05, **p < 0.01 and ***p < 0.001.
