Figure 1 | Scientific Reports

Figure 1

From: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)

Figure 1

ryr1a and ryr1b expression by fast and slow muscle in wild-type Pacific bluefin tuna, respectively. (A) RT-PCR analysis in larvae at 0, 4, 7, and 26 days after hatching (DAH), and in adult fast and slow muscle. RT-PCR shows that both mRNA are detected from hatching larvae to adulthood, and ryr1a and ryr1b genes specifically express in adult slow and fast muscle, respectively. (B) The distribution of ryr1a and ryr1b mRNA in the developing skeletal muscle of larvae at 7 and 26 DAH by in situ hybridisation. The insets indicate higher magnification of the slow developing muscle area. Cross-sections show that ryr1a is expressed by the lateral muscle of the vertebral column-slow developing muscle (arrowheads), whereas ryr1b is expressed by a large proportion of the muscle-both fast and slow developing muscles.

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