Figure 2 | Scientific Reports

Figure 2

From: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)

Figure 2

Design of ryr1b-TALENs and mutation rates of Pacific bluefin tuna embryos injected with Platinum TALENs. (A) ryr1b-TALENs designed to target exons 2 and 43 of the gene. The solid and open boxes represent coding and untranslated exon regions, respectively. The grey boxed sequences indicate the TALEN binding sites. (B) Mutation rates of the TALEN-injected hatched embryos analysed by heteroduplex mobility assays (HMA). The grey and white boxes represent the proportion of positive and negative embryos by HMA. The number of analysed embryos is indicated in brackets.

Back to article page