Figure 1
From: Synergy between B cell receptor/antigen uptake and MHCII peptide editing relies on HLA-DO tuning
DMfree/CLIPfreq measured in single cells is correlated with DO. (a) Contour plots of flow cytometric analysis (n = 4) of fixed/permeabilized T2 derivatives co-stained with fluorophore-conjugated antibodies specific for DM, DO, DR or CLIP, as indicated. (b) iFACS analysis (n = 3) of fixed/permeabilized T2DR4DMDO cells co-stained as in (a). Heatmap colors scaled to the index FICLIP of individual cells. (c) Index FIs of 3 representative cells as circled in (b) represented by stagger-offset histograms. (d) Comparisons of Index FIDO of individual T2DR4DMDO cells or DMfree/CLIPfreq determined by iFACS for individual cells from CLIPlo and CLIPhi groups. Data include 384 single cells. (e) Comparisons of mean FIDO from single clonal lines or DMfree/CLIPfreq determined by MFIs using flow cytometry for each line (CLIPlo vs CLIPhi). (f,g) Pairwise comparisons (paired t-test) of mean FIDO or DMfree/CLIPfreq or mean FICLIP of each line (indicated by colors) before (Day 0) and after (Day 7) downregulation of DO expression. (h) Overlay of histograms showing the CLIP levels of each line prior to (Day 0) or after (Day 7) downregulation of DO expression. ns: non-significant, p > 0.05; *p < 0.05, **p < 0.01, ****p < 0.0001.
