Figure 4
From: Unraveling FATP1, regulated by ER-β, as a targeted breast cancer innovative therapy
MDA-MB-231 and MCF7 cells exposed to PHTPP accumulate less FA. Cells were cultured in control (baseline culture medium represented by the dot line), control DMSO, estradiol and/or C18 conditions, in the presence or absence of PHTPP (A–D) or in the presence or absence of MPP and Fulvestrant (E–H). A flow cytometry analysis of Nile red staining was performed to evaluate the accumulation of neutral (λ 578 nm) and polar (λ 678 nm) lipids. Values are referenced to control/DMSO conditions within each cell line. Biological triplicates were tested, only adherent cells were analyzed and dead cells in culture media were discarded. Results are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. (*) represents the statistical analysis in relation to control condition and (#) represents the statistical analysis in relation to control DMSO condition. One-way ANOVA with Dunnett’s multiple comparisons test.
