Figure 6
From: Unraveling FATP1, regulated by ER-β, as a targeted breast cancer innovative therapy
Arylpiperazine 5k (DS22420314) interferes with FA uptake and cell viability. Cells were cultured in control, control DMSO, estradiol with and without C18 and exposed to arylpiperazine 5k (DS22420314). Nile red labelling was analyzed by flow cytometry in MDA-MB-231 cells (A) and MCF7 cells (B). Cell death assay was performed in cells exposed to arylpiperazine 5k (DS22420314) and compared to control and control DMSO, using flow cytometry using Annexin V and PI (C). Values are referenced to control/DMSO conditions within each cell line. Data are means of biological triplicates. Results are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. (*) represents the statistical analysis in relation to the control condition; (#) statistical analysis in relation to the DMSO condition; ($) statistical analysis in relation to the C18 condition. Biological triplicates were tested, adherent cells were dead cells in culture media were analyzed. The dot line defines the relative median fluorescence intensity for the control conditions. Multiple comparisons were performed using One-way ANOVA with Dunnett’s or Tukey’s test.
