Figure 2
Cell-specific analysis shows ubiquitous reduction of Epha4 expression in CAG-EphA4 mice and neuronal specific reduction in CAG-Thy1 mice. RNAscope in situ hybridization was performed to quantify EphA4 down regulation in neurons and in glial cells in the lumbar spinal cord of (A–C) CAG-EphA4 mice and in (D–F) Thy1-EphA4 mice. Epha4 expression was quantified as the number of Epha4 dots/µm2. Gene expression was quantified in (A,D) neurons (Syp positive) and in (B,E) glial cells (Syp negative). A total of 10–13 images of different ventral horns from 2 to 3 different mice lumbar spinal cords were analysed for every condition. Data represents mean ± SEM, and different conditions were compared with a two-tailed t-test: *** P < 0.001. (C,F) Representative images show the lumbar spinal cord ventral horn of EphA4, CAG-EphA4 and Thy1-EphA4 that were stained with probes against EphA4 and Syp. Hoechst was used as a counter stain for nuclei. Scale bar = 50 µm.
