Figure 2

Colonic shortening. (a) DSS treatment induced colonic shortening in all treatment groups, but relative to DSS-treated WT mice, was significantly greater in the WT DSS mice that had also received DMXAA. In contrast, this effect was significantly reduced in both the Tmem173^gt and the Tmem173^gt mice co-treated with the STING agonist. Black-filled dots are for WT, or WT + 5 or + 10 mg/kg DMXAA; white-clear dots are for the Tmem173^gt receiving similar treatments. Statistical significance between data sets was assessed by one-way ANOVA followed by Tukey’s multiple comparisons post-hoc test between all groups. Values are means ± SEM, n ≥ 8 mice per group, ***P < 0.001 longer relative to DSS-treated WT. Analysis of (b) crypt depth or (c) goblet cell numbers in the colons of the untreated WT and TMEM173^Gt mice. To assess crypt depth, 4 random locations from 6 untreated WT or TMEM173^Gt mice (n = 24 total) measurements were plotted. Upon statistical comparison, there were no significant differences in crypt height or goblet cell numbers when sections from untreated WT and Tmem173^gt mice were compared. Goblet cell counts represent the number of goblet cells per 200 um² area, n = 12, Student’s T-test was used to determine possible differences between data sets.