Table 2 Primers used in this study. Illumina forward and reverse overhang adaptors were attached to the primers for rbcL gene.

From: Unveiling the hidden genetic diversity and chloroplast type of marine benthic ciliate Mesodinium species

Primer

  

Remarks

Nuclear partial SSU-entire ITS-partial LSU for Mesodinium species

 

Sequence

  

MESO1200F

ATTCCGGTAACGAACGAGAC

 

Johnson et al.6

MESO1440F

AACTAGGAATGTCTCGTAAGC

 

Johnson et al.6

MESO28SR

AGACTTGGATGACTTTTATCACC

 

Johnson et al.6

Chloroplast rbc L gene for Cryptophytes

 

Overhang adaptor

Target sequence

 

CryprbcL646F

TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG

ATGAGATGGARAGAGCGTTTC

This study

Crypt_rbcLR2

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG

CAGTGRATACCACCWGAAGCWA

Modified from Johnson et al.6

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