Figure 1
From: Scribble co-operatively binds multiple α1D-adrenergic receptor C-terminal PDZ ligands
In situ affinity determination of α1D-adrenergic receptor C-terminal PDZ ligand:PDZ protein interactions. (A) Real-time biolayer interferometry (BLI) association/dissociation curve measuring binding of α1D C-terminus (α1D-CT) to purified scribble (SCRIB). Biotin-labeled α1D-CT was immobilized to streptavidin probes. Indicated concentrations of SCRIB were used as analytes. (Bio. = Biocytin, Diss. = Dissociation). (B–F) Quantified BLI binding data for biotin labeled α1D-CT binding to (B) SCRIB, (C) α1-syntrophin (SNTA), (D) human discs large MAGUK scaffold protein 1 (DLG1), (E) calcium/calmodulin dependent serine protein kinase (CASK), and (F) membrane palmitoylated protein 7 (MPP7). (G) Comparative analysis of BLI concentration-response curves for α1D-CT:PDZ protein association binding. (H) Reverse BLI assay of purified α1D-CT (analyte) bound to immobilized biotin-labeled SCRIB (probe). Data are presented as mean ± SEM, n = 3.
