Figure 2
Accumulation of Saposin D in Ppt1 ko retinas. (A) Immunohistochemical analyses revealed moderately and markedly elevated levels of saposin D in 45- (b) and 240-day-old Ppt1 ko retinas (d) respectively when compared to age-matched wild-type retinas (a and c, respectively). No fluorescence signal was detectable when wild-type (e) or mutant (f) retinal sections were incubated with secondary antibodies only. (B) Immunoblot analyses confirmed markedly increased levels of saposin D (arrowhead) in 240-day-old mutant retinas, and revealed similar levels of prosaposin (arrow) in both genotypes. GAPDH immunoblotting was performed as a loading control. Positions of molecular mass markers are indicated. Uncropped blots are shown in Supplementary Fig. 5. gcl: ganglion cell layer; inl: inner nuclear layer; ipl: inner plexiform layer; onl: outer nuclear layer. Scale bar in (f) (for a–f): 100 µm.
