Figure 4
The expression patterns of genes involved in biosynthesis of phenylpropanoid and lignin content of soybean roots. (a–c) Expression patterns of three representative genes involved in phenylpropanoid biosynthesis were determined by RNA-Seq and RT-qPCR at the 3, 6, 12 and 24-h submergence stresses. Normalization for RT-qPCR was performed using the ELF1B gene as an internal control. The gene expression levels in the submergence treatment samples are indicated by the log2FoldChange relative to that of the corresponding control samples. Error bars on the black boxes indicate the standard deviations of three biological replicates of RT-qPCR analyses. (a–c) genes related to phenylpropanoid biosynthesis. (d) The lignin content of soybean roots under the submergence treatment and without the submergence treatment. The sampling time points are 24, 96 and 192 h under the submergence stress. Error bars on the boxes indicate the standard deviations of three biological replicates. *P < 0.05, **P < 0.01; Student’s t-test. CK: Control; F: Flooding (submergence treatment).
