Figure 5

Expression patterns of four representative genes involved in other pathways were determined by RNA-Seq and RT-qPCR at the 3, 6, 12 and 24-h submergence stresses. Normalization for RT-qPCR was performed using the ELF1B gene as an internal control, and the gene expression levels in the submergence treatment samples are indicated by the log2FoldChange relative to that of the corresponding control samples. Error bars on the black boxes indicate the standard deviations of three biological replicates of RT-qPCR analyses. (a) Gene related to MAPK signaling pathway-plant; (b) gene related to carbon metabolism; (c) gene related to tryptophan metabolism; (d) gene related to isoflavonoid biosynthesis.