Figure 3

Six genes regulate cancer stem cell (CSC) phenotypes as well as the SP fraction. Sphere formation and single cell clonogenicity were assessed. (a–d) Newly generated SP cells harbor a CSC phenotype. (a,b) Sphere forming ability. Data from CH1-shcontrol and sh-target gene cells are shown in Fig. 3a; data from SKOV3-shcontrol and sh-target gene cells are shown in Fig. 3b. The number represents spheres larger than 200 µm (n = 6 replicates). (c,d) Single cell clonogenicity. Expanded clone colonies were counted and the proportion of cells capable of colony formation was calculated (n = 6 replicates). Data from CH1-shcontrol and sh-target gene cells are shown in Fig. 3c; data from SKOV3-shcontrol and sh-target gene cells are shown in Fig. 3d. (e–h) Overexpression of MSL3, VPS45, ITGB3BP, TLE2, ZNF498 decreased sphere formation and single cell clonogenicity. (e,f) Sphere formation. Data from A2780-control and ORF-target gene cells are shown in Fig. 3e. Data from IGROV1-control and ORF-target gene cells are shown in Fig. 3f. The number of spheres larger than 200 µm is depicted. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (g,h) Single cell clonogenicity. Expanded clones were counted and the proportion of cells capable of colony formation was calculated. Data from A2780-control and ORF-target gene cells are shown in Fig. 3g. Data from IGROV1-control and ORF-target gene cells are shown in Fig. 3h. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.