Figure 2
From: Concatenation of 14-3-3 with partner phosphoproteins as a tool to study their interaction
Oligomeric state of the 14-3-3-pB6 chimera. SEC profiles of either unphosphorylated (A) or PKA-phosphorylated (B) chimera loaded on a Superdex 200 Increase 5/150 column at different concentrations (indicated in mg/ml) and run at a 0.45 ml/min flow rate. MW values of the peaks were determined from column calibration. Dashed lines show the lowest concentration profiles scaled to the main peak of the highest concentration profiles for clarity. (C) Absolute MW values for the main two peaks of the phosphorylated chimera obtained by SEC-MALLS on a Superdex 200 Increase 10/300 column operated at a 0.5 ml/min flow rate. (D) Formation of chimera tetramers due to the domain swapping between two different chimera dimers. NTD IDRs – intrinsically disordered regions of the HSPB6 N-terminal domain.
