Figure 5 | Scientific Reports

Figure 5

From: The explant developmental stage profoundly impacts small RNA-mediated regulation at the dedifferentiation step of maize somatic embryogenesis

Figure 5

Relative abundances of selected sRNA and their corresponding targets during callus induction from zygotic embryos collected at distinct developmental stages. sRNA and mRNA abundances were analyzed by qRT-PCR in Tuxpeño VS-535 immature embryos (IE15, IE23) and mature embryos (ME) used as explants and the corresponding induced one-month callus tissues (C15, C23, CM). Accumulation of selected sRNAs (upper graphs, 1st row) and their targets (lower graphs, 2nd and 3rd rows) are shown in similar bar color. (a) miRNAs and targets; (b) tasiARF and targets. Fold change represents abundances relative to IE15. sRNAs were normalized by U6 snRNA and targets by 18S rRNA internal controls. (c) Heat maps comparing differential accumulation of selected sRNAs by massive sequencing and qRT-PCR according to the value of log2FC.

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