Figure 5

Suppression of cardiomyocyte generation by inhibiting entry or intracellular release of Ca²⁺. (A) Effects of nicardipine (10 µM) and 2-APB (3 µM) on the growth of spontaneously beating cardiomyocyte clusters after 3 weeks culture; total areas (panel a), average areas (panel b) and numbers (panel c) of spontaneously beating clusters (mean ± S.E.M., n = 6) are illustrated. B, C, effects of nicardipine (10 µM) and 2-APB (3 µM; panel B) and tetrodotoxin (10 µM, panel C) on Nkx2.5, cTnT and RYR2 mRNA expression after 2 weeks of SANC culture (n = 6), as estimated using quantitative RT-PCR. Bars denote mean ± S.E.M. (n = 6); *^,†P < 0.05; **^,##P < 0.01; ^{***,†††,###}P < 0.001. Symbols denote significant differences from corresponding control values (Dunnett’s test).