Figure 5 | Scientific Reports

Figure 5

From: Unbiased peptoid combinatorial cell screen identifies plectin protein as a potential biomarker for lung cancer stem cells

Figure 5

Plectin knockdown decreases CSC characteristics both at genotypic and phenotypic levels. (A–D) Plectin siRNA knockdown cultures from four cell lines: (A) H358, (B) H1693, (C) H460, and (D) H1975, have decreased expression of PLEC, ALDH1A3 and SOX2, where detectable, while H1693, H460, and H1975 also show decrease in CD44. Error bars represent standard deviation between triplicates. (E,F) Knockdown of plectin decreases colony formation potential in H358, H1693, H460,and H1975 cells. (E) Visualization of colony formation. (F) Quantification of colony count. (G,H) Knockdown of plectin decreases the mobility and scratch healing in H358, H1693, H460, and H1975 cell lines, incubated for 60 hours with siRNA (36 hours before scratch and 24 hours after). Error bars represent standard deviation between duplicates. *Represents p value < 0.05.

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