Figure 2

Protein stability assessment for YidC2 with and without the C2 loop. (A) Root mean square deviation (RMSD) time series and (B) root mean square fluctuation (RMSF) estimations for YidC2 with (blue) and without (red) the C2 loop obtained from 2-μs simulations. RMSD analysis indicates that the absence of the C2 loop destabilizes the protein and RMSF analysis indicates that the presence of the modeled C2 loop (residues 195 to 216) significantly stabilizes the entire protein, particularly in the C1 loop region (residues 84–133).