Figure 1

E.G7 lymphoma cells suppress the activation of CD4⁺ T cells. CD4⁺ OT-II TCH (1.25 × 10⁵) were stimulated with JAWS II DC (5 × 10⁴) and OVA323 (0.1 mg/ml) in the presence or absence of E.G7 cancer cells (2.5 × 10⁴) for 24 h (1 ml total volume/well in duplicate). Wells including: E.G7 + DC + OVA323; E.G7 + OT-II TCH; and E.G7 + DC + OT-II TCH served as controls. At the completion of incubation, culture supernatants were collected and assessed for IL-2 production by ELISA. For each bar, the data represent the mean + SD of n = 6 of three independent experiments (n = 18/condition). ***p-value ≤ 0.0001 comparing OT-II TCH IL-2 production in the presence and absence of E.G7 cancer cells.