Figure 1
From: HspX promotes the polar localization of mycobacterial protein aggregates

HspX promotes polar localization of protein aggregates. (a) Fluorescence microscopy images of wild-type M. smegmatis expressing dual fluorescent GLR (top panel) or mutated GLR103 proteins. Images illustrative of >300 individual cells. (b) Fluorescence microscopy of wild-type M. smegmatis (WT), hspX-deleted M. smegmatis (hspX KO) and complemented (hspX KOā+āhspX) strains expressing GLR103. Images illustrative of >100 cells. (c) Analysis of red channel fluorescence intensity from GLR103 across the normalized cell length of images represented in (b) of the three strains. The y-axis represents relative red fluorescence along the cell axis compared with the mean fluorescence intensity of the cell being analyzed (see Methods). The symbol (n) represents number of individual cells analyzed.