Table 1 Plasmids used in this study.

pUVtetOR

Episomal mycobacterial expression vector with tet^ON inducible system, Hyg^R

Ref.²

pUVtetOR-GFP-linker-RFP (GFP-mRFP)

pUVtetOR containing GFP translationally fused with RFP through a long flexible linker (GGSGGGGSGGGSSGG), Hyg^R

This Study

pUVtetOR-GLR-D103N (GFP-D103N-mRFP – also called GLR103)

Mutation of D103N on GFP of pUVtetOR-GFP-linker-RFP, Hyg^R

This Study

pUVtetOR-GLR103-HspX

Vector expressing both HspX and GLR103 reporter, Hyg^R

This Study

pUVtetOR-HspX-GFP

Vector expressing HspX C-terminal fused with GFP, Hyg^R

This Study

pUVtetOR-ΔN HspX-GFP

Vector expressing N-terminal truncated HspX C-terminal fused with GFP, Hyg^R

This Study

pUVtetOR-ΔC HspX-GFP

Vector expressing C-terminal truncated HspX C-terminal fused with GFP, Hyg^R

This Study

pUVtetOR-ΔNΔC HspX-GFP

Vector expressing N-terminal, C-terminal truncated HspX C-terminal fused with GFP, Hyg^R

This Study

pML1357

Integrative mycobacterial expression vector, Hyg^R

Addgene

pSML1357

Integrative mycobacterial expression vector with selection marker changed from original Hyg^R to Strep^R

This Study

pSML1357-HspX-mcherry

Vector expressing HspX C-terminal fused with mcherry, Strep^R

This Study

pSML1357-ΔN HspX-mcherry

Vector expressing N-terminal truncated HspX C-terminal fused with mcherry, Strep^R

This Study

pSML1357-N35 HspX-mcherry

Vector expressing N-terminal HspX C-terminal fused with mcherry, Strep^R

This Study

pUVtetOR-GLR103-SepF-linker-HspX

Vector with ATC induced expression of GLR103 reporter and SepF-linker-HspX fusion construct. Hyg^R

This Study

pSML1357-Msmeg_6339-mApple

Vector expressing mApple C-terminal fused with Msmeg_6339, Strep^R

This Study

pSML1357-murA-mApple

Vector expressing mApple C-terminal fused with murA, Strep^R

This Study

pSML1357-tuf-mApple

Vector expressing mApple C-terminal fused with tuf, Strep^R

This Study

pSML1357- Msmeg_1028-mApple

Vector expressing mApple C-terminal fused with Msmeg_1028, Strep^R