Table 2 Primers used in this study.
From: HspX promotes the polar localization of mycobacterial protein aggregates
Name | Sequence 5ā²-3ā² |
|---|---|
GFP-PacI-F | AATGTTAATTAAGAAGGAGATATACATCATGTCGAAGGGCGAGGAGCTGTTC |
GFP-linker-R | GAGCCGCCGCCCGAGCCGCCGCCGCCCGAGCCGCCCTTGTACAGCTCGTCCATGCC |
mRFP-linker-F | GCGGCGGCTCGGGCGGCGGCTCGTCGGGCGGCGCCTCCTCCGAGGACGTCATCAAG |
mRFP-EcoRV-R | AATCTTGATATCTTAGCGCCGGTGGAGTGGCGG |
GFP-D103N-Mut-F | CCTTCAAGGACAACGGTAACTACAAGAC |
GFP-D103N-Mut-R | GTCTTGTAGTTACCGTTGTCCTTGAAGG |
BamHI-HspX-F | AACACGGATCCAATGACCAAACTTCCTGAAC |
HspX-GFP-R | GGTGGCCACCGGCGGGTCCGGGCTGACGGTCTCCACCGCG |
HspX-GFP-F | GACCCGCCGGTGGCCACCATGTCGAAGGGCGAGGAGC |
HindIII-GFP-R | TTCGCTAAGCTTCTACTTGTACAGCTCGTCC |
HspXKO-1 | ACCGTGCGGCACGGGGAGAT |
HspXKO-2 | GGCCTCTCGAAGCGGTCCTCCTCACTCGTAGG |
HspXKO-3 | AGGAGGACCGCTTCGAGAGGCCTATAACTTCG |
HspXKO-4 | CGGCCATGGCGAAGTACTTCTAGACTCGAGATAACTTCG |
HspXKO-5 | TCTAGAAGTACTTCGCCATGGCCGTGAACA |
HspXKO-6 | GCCCGGTGCCCATGGAC |
RBS-HspX-GFP-F | AAGGAGATATACATCATGACCAAACTTCCTGAACGATCACGAG |
pUVtet-ā³N35HspX-GFPa | ATAGGCTCTGGGAGTACCCG GACCACATCATCCGGATCGAGG |
pUVtet-ā³N35HspX-GFPb | GAAAAGTTCTTCTCCTTTACT CGGGCTGACGGTCTCCACCGCG |
pUVtet-ā³N35HspX-GFPc | CGGTGGAGACCGTCAGCCCG AGTAAAGGAGAAGAACTTTTCAC |
pUVtet-ā³N35HspX-GFPd | CCCCAATTAATTAGCTAAA TCAACATTTGTATAGTTCATC |
pUVtet-HspX-GFPe | CGGGTACTCCCAGAGCCTATCTATC |
pUVtet-HspX-GFPf | TTTAGCTAATTAATTGGGGACCC |
pUVtet-ā³C HspX-GFPa | ATAGGCTCTGGGAGTACCCG ATGACCAAACTTCCTGAACGATC |
pUVtet-ā³C HspX-GFPb | GAAAAGTTCTTCTCCTTTACT GAACCCGAGGCAGCCGCCGAGAAG |
pUVtet-ā³C HspX-GFPc | CCCGAGGCAGCCGCCGAGAA AGTAAAGGAGAAGAACTTTTCAC |
pUVtet-ā³C HspX-GFPd | CCCCAATTAATTAGCTAAA TCAACATTTGTATAGTTCATC |
pUVtet-ā³Nā³CHspX-GFPa | ATAGGCTCTGGGAGTACCCG GACCACATCATCCGGATCGAGG |
pUVtet-ā³Nā³CHspX-GFPb | GAAAAGTTCTTCTCCTTTACT GAACCCGAGGCAGCCGCCGAGAAG |
pUVtet-ā³Nā³CHspX-GFPc | CCCGAGGCAGCCGCCGAGAA AGTAAAGGAGAAGAACTTTTCAC |
pUVtet-ā³Nā³CHspX-GFPd | CCCCAATTAATTAGCTAAA TCAACATTTGTATAGTTCATC |
mcherry-wtHspX-R | GACGTCCTCGGAGGAGGCCGGGCTGACGGTCTCCACCGCGACA |
wtHspX-mcherry-F | GTGGAGACCGTCAGCCCGGCCTCCTCCGAGGACGTCATCAAGG |
HindIII-mcherry-R | CCCAAGCTT TTA ACTGGATCCGCTAGATCCCTGGGAGCC |
mcherry-N35HspX-R | GAC GTC CTC GGA GGA GGC ACC GAA AAC CGG GCG GAT CGA GGC C |
N35HspX-mcherry-F | ATC CGC CCG GTT TTC GGT GCC TCC TCC GAG GAC GTC ATC AAG G |
PacI-RBS-ā³NHspX-F | GCC TTA ATT AAG AAG GAG ATA TAC ATC GAC CAC ATC ATC CGG ATC GAG GAC GA |
PacI-SepF-F | CCTTAATTAACAGAAAGGAGGTTAATAATGAGCACACTGCATAAGGTCAAGGCC |
SepF-linker-HspX-R | TTGGTGCCGCCCGACGAGCCGCCACGGTAGGAGTAGAAGCCCGCCTCGG |
Linker-HspX-F | GGCGGCTCGTCGGGCGGCACCAAACTTCCTGAACGATCACGAGCACGC |
HspX-HindIII-R | CCCAAGCTTTTACGGGCTGACGGTCTCCA |
GLR103-SepF-F | ACCGGCGCCTAAGATCAGAAAGGAGGTTAATAATGAGCAC |
HspX-Ptet R | GGTCCCCAATTAATTAGCTAAAGCTTGATTTACGGGCTGACGGTCTC |
mApple-F | CTA GCT AGC AGC AAG GGC GAG GAG AAT AAC ATG GCC ATC ATC AAG GAG TTC ATG CGC |
mApple-R | CCC AAG CTT TTA GGT CGA GTC CAG GCC CAG CAG CGG GTT CGG GAT CGG CTT GCC CTT GTA CAG |
Msmeg_6339-F | CCTTAATTAAGAAGGAGATATACATCatggagtcgaacccaccgagtgccgtcgtcga |
Msmeg_6339-R | CTAGCTAGCgggcgtgacgatgctgaagttcgggt |
murA-F | CCTTAATTAAGAAGGAGATATACATCgtgagcgagcgtttcgtggtgaccggtggcaa |
murA-R | CTAGCTAGCcgagcttactctctcgatctcggctc |
tuf-F | CCTTAATTAAGAAGGAGATATACATCgtggcgaaggcgaagttcgagcggacgaagcc |
tuf-R | CTAGCTAGCcttgatgatcttggtgacgcggccgg |
