Figure 3
Effect of proliferation and invasion in OSCC cell lines by co-culture with CM of TAM subsets. (A) Scheme and representative image for the co-culture of HSC-2 cells and CM of TAM subsets for 4 days (n = 3 for each subset). Cells were cultivated as described in Materials and Methods. (B) Viability of HSC-2 cells co-cultured with CM of TAM subsets with/without anti-EGFR antibody (n = 3 for each subset). (C) Invasion activity of HSC-2 cells co-cultured with CM of TAM subsets (n = 3 for each subset). Scale bars, 100 μm. Statistically significant differences between groups were determined by Kruskal-Wallis test (*P < 0.05) and Mann-Whitney U-test (†P < 0.05, ††P < 0.01, †††P < 0.001).
