Figure 2
From: Adipocyte PU.1 knockout promotes insulin sensitivity in HFD-fed obese mice
Improved glucose tolerance and insulin sensitivity in PU.1 AKO mice. (A) Glucose tolerance test (GTT) (B) Area under curve (AUC) of GTT. (C) Fasting insulin after 10 wks of HFD, and (D) insulin tolerance test after 11 wks of HFD in fl/fl and PU.1 AKO. (E) Body weight, (F) glucose infusion rate (GIR), (G) basal and clamp hepatic glucose production (HGP), (H) percent HGP suppression, (I) percent free fatty acid (FFA) suppression, (J) basal glucose disposal rate (GDR), and (K) insulin stimulated (IS)-GDR during hyperinsulinemic-euglycemic clamp after 12 wks HFD. Insulin-stimulated AKT phosphorylation in (L) liver, (M) eWAT, and (N) skeletal muscle 14 wk after HFD. (O) Representative H&E staining of paraffin embedded liver sections. (P) Triglyceride (TG) concentration in livers from HFD-fed fl/fl and PU.1 AKO mice. Expression of genes associated with pro-inflammation (Q) and anti-inflammation (R) in livers from HFD-fed fl/fl and PU.1 AKO mice. Values are expressed as mean ± SEM, n = 7–10 per group, *p < 0.05 by t-test.
