Figure 3
From: Adipocyte PU.1 knockout promotes insulin sensitivity in HFD-fed obese mice
PU.1 negatively affects adipocyte metabolism. (A) Relative mRNA expression of adipogenic-associated genes in eWAT from fl/fl and PU.1 AKO mice after 14 wk HFD feeding and (B). Adipocyte diameter was quantified and sorted by bins with Lowess curve fitting in both f/f mice (B) and PU.I AKO mice (C). (D) Epididymal adipose weight. Glucose uptake, as a percent of basal 3H-2-deoxy-D-glucose uptake, in (E). Cultured primary adipocytes from eWAT from HFD-fed fl/fl and PU.1 AKO mice. Plasma concentrations of (F). leptin, (G). Total PAI-1, (H). Adiponectin, (I). Resistin, (J). Free fatty acids (FFA), and (K). Triglycerides (TG) in fl/fl and PU.1 AKO mice after 14 wk HFD feeding. Values are expressed as mean ± SEM, *p < 0.05 by t-test. All groups are significantly different from each other for each gene analyzed in B by 2-way ANOVA followed by Tukey’s post-hoc test. N = 10–14 per group in (A) and (F–K), n = 6–8 per group in (B–E).
