Figure 2

Crystal structure of the aP1-CTD•aEF1A•GTP•aPelota complex. (A) Overall structure of the GTP-bound complex _ApeP1C17•_ApeEF1A•GTP•_ApePelota. _ApeEF1A•_ApePelota, _ApeP1C17, and GTP are represented by surface, ribbon, and stick models, respectively. Colors in _ApeEF1A are as follows, domain 1 (pink), domain 2 (orange) and domain 3 (yellow); _ApePelota is blue; aP1 is green. The N- and C-termini of _ApeP1C17 are indicated as ‘N’ and ‘C’, respectively. (B) Structural comparison of _ApeEF1A•GTP in the complex described here with _PhoEF1A•GDP [PDB ID: 3WY9]¹² superposing domains 2 and 3. The colors of domains of aEF1A are as in (A), except for domain 1 of _PhoEF1A•GDP (grey). (C) Structure of the binding interface between _ApeP1C17 and _ApeEF1A in the _ApeP1C17•_ApeEF1A•GTP•_ApePelota complex. _ApeEF1A and _ApeP1C17 are represented by a ribbon model, and the amino acid residues of _ApeEF1A and _ApeP1C17 that participate in the interaction by stick models. The color coding is the same as in (A). (D) Schematic diagram of the interactions between _ApeP1C17 and _ApeEF1A. The van der Waals contacts and hydrogen bonds are represented by black and red lines, respectively. Amino acid residues and numbering are for A. pernix samples, and those in parentheses are for P. horikoshii samples.