Figure 4 | Scientific Reports

Figure 4

From: Switch of the interactions between the ribosomal stalk and EF1A in the GTP- and GDP-bound conformations

Figure 4

Effects of the amino acid substitutions in aEF1A on aEF1A/aEF2-dependent polyphenylalanine synthesis. P. furiosus 70S ribosomes were incubated with P. horikoshii aEF2 and P. horikoshii wild-type aEF1A (bar 2) or each of the aEF1A mutants (bars 3–9) in the presence of poly(U) and E. coli [14C]Phe-tRNA. The amount of polyphenylalanine synthesized in the 10 min reaction was assayed as described in Materials and Methods. Error bars represent SD values (n = 3).

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