Figure 5
Model of aP1•aEF1A•GTP•aPelota complex docking with the 70S ribosome. Colors are as follows: 50S (white), 30S (light yellow), stalk complex (green), aPelota (blue), aEF1A domain 1 (pink), aEF1A domain 2 (orange) and aEF1A domain 3 (yellow). In this docking model, we superimposed the aP1•aEF1A•GTP•aPelota complex described in this paper onto the bacterial 70 S•EF-Tu•GDPCP•aminoacyl-tRNA complex [PDB ID: 4V5G]22 (Voorhees et al. 2010). Subsequently the aP0•aP1 stalk complex core from Pyrococcus horikoshii [PDB ID: 3A1Y]10 was superimposed onto the model. The aP0•aP1 stalk complex core and aP1C17 peptide bound to aEF1A were connected by the flexible hinge region of aP1. Other hinge regions of aP0 and aP1 were modeled arbitrarily.
