Figure 4
From: Fucosylated inhibitors of recently identified bangle lectin from Photorhabdus asymbiotica
(a) Thermodynamic profiles for interaction between PHL and fucoligands determined by isothermal titration calorimetry at 25 °C. (b) Selected ITC curves of PHL (50 μM) titration by azide 1 (5 mM), difucoside 5 (5 mM), dendrimer 9 (1.5 mM) and dendrimer 10 (1.5 mM). 20 injections of 2.0 μl of fucoclusters were added every 240 s to a PHL-containing cell. The lower plots show the total heat released as a function of total ligand concentration for the titration shown in the upper panels. Due to low affinity of PHL to monovalent compounds, n, ΔH and ΔS values may suffer from higher inaccuracy.
