Figure 3
Ciliogenesis is essential for HP induced mineralizeation in SHED. (a) Immunostaining and quantification of cilia formation. The SHED were cultured in the presence or absence of 2 μM chloral hydrate (CH) and with or without the HP by the medium height of 5 cm (H: 5cm) for 6 hrs. The acetylated α-tubulin-positive cells were counted in twenty randomly selected fields of view under an inverted microscope (20X magnification). The bar graph shows the percentage of acetylated α-tubulin-positive cells from total cells with nuclear staining. Scale bar: 25 μm. (b) Alizarin Red S staining for odontoblast differentiation in SHED cultured in induction media and 2 μM chloral hydrate (CH) for 7 days. Cells were also subjected with or without the HP by the medium height of 5 cm (H: 5 cm). The Alizarin Red-positive areas were quantified using ImageJ. Scale bar, 150 μm. The data, as shown, are the representative of three independent experiments with similar results, and error bars indicate standard deviations. Statistical analysis was performed using analysis of variance.
