Figure 7
Nuclear translocation of RUNX2 is regulated by the activation of PIEZO1 in SHED. (a) Imunostaining analysis for nuclear translocation of RUNX2. SHED were cultured with or without HP by the medium height of 5 cm (H: 5cm) or 5 μM Yoda1 for 24 hrs. Green, RUNX2; blue, DAPI; Scale bars, 200 μm and 50 μm. (b) Nuclear translocation of RUNX2 in PIEZO1-specific siRNA transfected cells. The PIEZO1-specific siRNA transfected cells SHED were replaced and cultured with or without HP by the medium height of 5 cm (H: 5cm) for 24 hrs. RUNX2 nuclear translocation was assessed and quantified as the percentage of the DAPI nuclei-positively stained cells to the total cells. Three different siRNAs specific to PIEZO1 were tested, and a similar result was obtained. The data are representative of three independent experiments with similar results and error bars indicate standard deviations. Statistical analysis was performed using analysis of variance (**p < 0.01).
