Figure 5

Tissue damage induced by irradiation is a pre-requisite for RIG-I induced immunosuppression. (A) Scheme of experimental setup: Balb/c mice were treated with 3pRNA or were left untreated. Four days later, splenic CD11c⁺ cells were isolated and cocultured with allogeneic CD4⁺ or CD8⁺ T cells derived from C57BL/6 mice. Proliferation and IFN-γ production were analyzed on day 3 (CD8⁺ T cells) or 5 (CD4⁺ T cells) after onset of the mixed lymphocyte reaction (MLR). (B) Percentage of proliferated and IFN-γ⁺ cells of all CD4⁺ T cells on day 5 after onset of the MLR (left panel). Percentage of proliferated and IFN-γ⁺ cells of all CD8⁺ T cells on day 3 after onset of the MLR (right panel). Pooled data of two independent experiments. (C) FACS gating of data presented in B. FACS plots of CD4⁺ T cells (left panel) and CD8⁺ T cells (right panel). Representative data from one of two experiments. Donor animal numbers per group (n) are depicted. Data were analyzed using two-tailed unpaired t test. Significance was set at p values < 0.05, p < 0.01 and p < 0.001 and was then indicated with asterisks (*^,** and ***). Data are presented as mean ± S.E.M.