Figure 2
From: Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis
Obtaining P. brasiliensis PbTufM aRNA isolate. (A) Agarose gel electrophoresis to confirm the transference of HPH gene by ATMT to PbEV (PbWT transformed with empty vector) and PbTufM aRNA (PCR amplification yielded a 1,000 bp internal fragment), NC: negative control of PCR reaction, MW: 1Kb DNA ladder (GeneDireX). (B) Expression levels of PbTUFM assessed by Real-Time PCR in PbWT, PbEV, and PbTufM aRNA yeast cells after being subcultured for 1 and 12 months (gene expression levels obtained by RT-PCR were normalized to the level of expression of the internal reference, β-tubulin; ***p < 0.0001 compared with PbWT and PbEV.
