Figure 3
From: Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis
Silencing of PbTUFM and consequences for P. brasiliensis yeast cell growth, viability and morphology. Cellular morphology of exponentially grown yeast cells from PbWT, PbEV, and PbTufM aRNA stained with Calcofluor white and visualized by fluorescence microscopy. Magnification of 40× and white bars correspond to 20 μm (A). Growth curves and viability of P. brasiliensis isolates PbWT, PbEV, and PbTufM aRNA were evaluated by counting of yeasts by optical microscopy using Neubauer chamber and spotting in BHI medium (nutrient rich-mediun) (B,E), McMM medium with glucose (C,E) and McMM medium with glycerol (D,E).
