Figure 4
From: Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis
Effect in global translation rate due to PbTufM reduction. The rate of translation in PbWT, PbEV, and PbTufM aRNA was measured as the rate of incorporation of puromycin into newly synthesized polypeptides (by puromycin-labeled assay). (A) Gel stained with CBB (Coomassie Brilliant Blue) to demonstrated equal protein loading, (B) Ponceau-S staining showing total proteins levels and (C) western blotting with anti-puromycin antibody, (D) relative band intensity of newly synthesized proteins with puromycin incorporated in all strains. p < 0.0001 when PbTufM aRNA compared with PbEV and PbWT.
